Volume 3 Article informations Analysis of the grapevine fanleaf diseaseand genetic diversity of tunisianGFLV Isolates Besma Mrabet Saamali b Asma Toumi a , Amira Mougou-Hamdane a , Ahmed Sahbi Chakroun b and Sadreddine Kallel a Abstract Genetic analyses of grapevine fanleaf virus (GFLV) was done on the basis of the coat protein gene sequences from 11 isolates detected in 3 tunisian grapevine growing regions:Grombalia, Rafraf andTakelsa and amplified with a newly designed primer pairGT 1076/GT 1826.The amplified region of 2CP gene(750pb) wascaracterised by RT-PCR. After sequencing, alignement shows a variability within the obtained isolatesranged from 8.1 % to 15.4 % at the nucleotide level . Based on the result of genetic divergence of 312 pb long part of the 2CP gene, the level of variation found in this study suggests that this rate of diversity is common for each gene of the coat protein. Key words: Grapevine, GFLV, symptomology, genetic diversity.